Development and Validation of UV Spectroscopic method for estimation of Ellagic acid in Herbal capsule used for the treatment of Diabetes

Abstract

The UV spectroscopy fingerprinting method was developed via estimation of ellagic acid for, marketed formulation and raw material Eugenia jamboloma. Ellagic acid was found to follow Beer Lambert’s law in concentration range 2-12μg/ml at λmax 280 nm. The correlation coefficient (r2) was calculated, where the r2 value 0.996 indicates the good linearity between the concentration and absorbance. The estimation of ellagic acid content of formulated capsules (one marketed formulation) and Eugenia jamboloma was carried out separately. The concentration of ellagic acid present in raw material was determined in Eugenia jamboloma and in marketed formulation MCM. From the validation data it was observed that the present method of spectrophotometric determination of ellagic acid is simple, precise, accurate and suitable for routine analysis of ellagic acid in selected formulation.