Evaluation of tissue level antioxidant activity of Premna serratifolia leaf in paracetamol intoxicated wistar albino rats

Abstract

Antioxidant activity of methanolic extract of Premna serratifolia Leaf was determined in Paracetamol intoxicated Wistar albino rats.  The experiment was comprised of five groups as Healthy control group, Disease control (paracetamol treated), Positive control group (paracetamol+ silymarin), Treatment groups (test drug + paracetamol) lower dose (100mg/kg b.wt.) and higher dose (200mg/kg b.wt.), having six animals in each group.  The animals were maintained in the standard laboratory conditions and the test extract was administered p.o.   The antioxidant enzymes superoxide dismutase, catalase, glutathione peroxidase were evaluated in the blood samples and as well as in the isolated tissue samples of liver, kidney and heart.  The disease control group showed decreased status of antioxidant enzymes in blood and tissue samples.  But the elevated levels of SOD, catalase, glutathione were observed significantly (*p<0.05, *p<0.01) in liver, kidney and heart tissue samples and blood samples of the extract administered groups.  The activity was found to be dose dependent.  The overall efficacy of the extract is comparable with the standard drug silymarin.  The study revealed that the methanolic extract of Premna serratifolia was found to have potential antioxidant activity in the animal model system.  The further research on characterization of functional molecules has to be initiated.