Separation of photosynthetic pigments in Spirogyra specis by means of thin layer Chromatography from Sola lake, Ahmedabad, Gujarat

Abstract

The present study of three deferent sessons summer, monsoon and winter sample collaction from Sola lake, Ahmedabad surfes water this lab was to determine in Spirogyra specis the rate of flow values (Rf=distance of component/distance of solvent) for ceratin pigment found in chlorophylls, xanthophylls and carotene.chllorophyll, we observed the following four colours: yellow, Blue- green, Green and Carotene is red-orange the both of our experiments traveled a distance of 10.0cm. We obtained the following Rf avlues for the chlorophyll-a 0.22 to 0.32, chlorophyll-b 0.12 to0.19, xanthophyll 0.70 to 0.74 and carotene is 0.90 to 0.93 present.