A study on optimization of Penicillium chrysogenum culture media in Solid state Fermentation process for Pectinase enzyme production

Abstract

The present research finding has shown that optimization of Penicillium chrysogenum culture media in solid state fermentation process for pectinase enzyme production. Four moulds isolated from garden soil samples of Andhrahalli area, Bangalore, Karnataka, India and were screened for pectinolytic enzyme production when grown on pectin containing (YPSS) solid media. Penicillium chrysogenum was selected based on clearance zones and pectinase enzyme production was carried out in submerged fermentation. Enzyme production by Penicillium chrysogenum was higher at pH 6.0 and a temperature of 30°C using sucrose and ammonium sulphate as carbon source and nitrogen source, respectively. The maximal activity of P. chrysogenum pectinase was at 60°C, pH 5.0 and was thermostable up to 70°C. Manganese sulphate had greatest effect on pectinase enzyme activity.