Studies on phyto chemical constituents, quantification of total phenol, alkaloid content and In-vitro anti-oxidant activity of Coccinia cordifolia

Abstract

In the present study we have extracted dried plant of Coccinia cordifolia in hexane, ethyl acetate, ethanol (70%v/v) and methanol which was collected from Araku, Visakhapatnam Dist, Andhra Pradesh, India. These extracts were checked for its antioxidant and their phyto chemical constituents. The whole plant of Coccinia cordifolia revealed the presence of steroids, triterpenoids, glycosides, saponins, tannins, alkaloids, saponins, phenols and carbohydrates but extract of Coccinia cordifolia give the negative results for quinines and oils. The methanolic extract has more phenolic content than other extracts and the ethanol (70%v/v) extract has more alkaloid content than other extracts. The selected plant extracts were produced concentration dependent percentage inhibition of superoxide radical and produced maximum activity at a concentration of 160 µg and there after the percentage inhibition were raised gradually to its maximum level with higher concentrations. Among the four types of C.cordifolia extracts, the methanolic extract showed better activity than remaining extracts at 160 µg concentrations.