Formulation and In-vitro evaluation of controlled polyherbal microemulsion for the treatment of diabetes mellitus

Abstract

Aim of present study was to formulate and evaluate microemulsion for oral drug delivery of Tinospora cordifolia,
Momordica charantia and Trigonella foenum graecum to enhance bioavailability of extracts. Oil–in-water
microemulsions were prepared by high mixer homogenizer method. Microemulsion was formulated using Liquid
paraffin oil as oil phase; Tween 80 and Span 20 were used as surfactant and cosurfactant respectively, on the basis
of solubility studies. Aqueous phase titration method was used to construct phase diagrams. Formulations were
selected from phase diagrams. Prepared microemulsions were subjected to various parameters like: thermodynamic
stability tests, drug content, viscosity, percent transmittance, electroconductivity, droplet size and in-vitro release
studies. Results showed that all the formulations had good stability. Viscosity of F1 optimized formulation was
found to be 4.86cPs, percent transmittance was found to be 98.32% and drug content was found to be 89.62%,
97.78% and 99.75% of Momordica charantia, Trigonella foenum graecum and Tinospora cordifolia respectively.
Particle Size through SEM was found to be 861nm-1.18µm. Optimized formulations were successful in control
release for 18 hours. Formulation F1 showed 89.28%, 92.31%, 99.03% drugs release of Momordica charantia,
Trigonella foenum graecumand Tinospora cordifolia respectively.