Stability Indicating Assay for Simultaneous Estimation of Aspirin and Omeprazole in Bulk by Validated RP-HPLC Method

Abstract

The aim of the present investigation is to study Stability Indicating Assay for Simultaneous Estimation of Aspirin and Omeprazole in Bulk by Validated RP-HPLC Method. The λmax of the two ingredients i.e. Aspirin and Omeprazole, were found to be 240 nm and 270 nm respectively in mobile phase as solvent system. The isobestic point for the drugs was observed at 288 nm. 10 mg of Aspirin and Omeprazole was weighed accurately and transferred into 100 ml volumetric flask. About 10 ml versatile phases was inserted and sonicated to dissolution. The volume was made up to the mark with same solvent. The different HPLC conditions were employed to conclude out the optimum condition for superior extraction of drugs. Linearity range was observed as 0-60 μg/ml for Aspirin. The correlation coefficient was observed as 0.999, the slope was found to be 11904 and intercept was observed as 12043 for Aspirin. Linearity range was observed as 0-40 μg/ml for Omeprazole. The correlation coefficient was observed as 0.999, the slope was observed as 15639 and intercept was observed as 2119 for Omeprazole.
The mean recoveries were observed as 100.41, 100.66 and 100.963% for Aspirin and 100.75, 100.59 and 100.05% for Omeprazole. As results are within the limit of 98-102%, the approach developed passes the test and hence can be reused. Based on spike purity results, acquired from the investigation of specimens utilizing depict approach, it can be finalized that due to lack of co-eluting spike along with the main spike of Aspirin & Omeprazole showed that the developed method is specific for the synchronal calculation of Aspirin & Omeprazole in the bulk.